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Ultrastructure of Marine Organisms

Course Numbers: OCMB - 6530, MEVS - 5145, and BMME - 7080

This course will examine the ultrastructure of marine organisms and range in focus from bacteria to fish. Lectures and labs will be conducted to examine scanning and transmission electron microscopy (SEM and TEM) of tissue and cells from many marine groups. Lectures will include discussion of basic theory of electron optics and include elemental analysis (EDS). The preparation of specimens for SEM and TEM including fixation, dehydration, embedding, sectioning, and staining will be discussed in lectures. Additionally, examination of the application of electron microscopy to the field of biomineralization will be discussed. In the labs students will prepare their own research specimens for SEM and/or TEM following the protocols discussed in lecture. Students will conduct presentations and submit a term paper at the end of the semester.

Learning Objectives

Students will:

  • Obtain a basic understanding of electron optics
  • Obtain skills required for preparation of specimens for SEM and TEM will be acquire
  • Acquire the knowledge required for operation of the SEM and TEM
  • Use techniques for collection of SEM/TEM images and image processing
  • Understand the relationship between ultrastructure and biomineralization

Recommended - Principles and Techniques of Electron Microscopy: Biological Applications. M. A. Hayat (ed.).2000. 4th ed., CRC Press, Inc., Boca Raton, Florida

Winter 2016 Syllabus

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